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ab189034  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc ab189034
    Ab189034, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1774 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab189034/Phospho-S6+Ribosomal+Protein+(Ser240%2F244)+Antibody/pmc08431545-149-85-88
    Average 96 stars, based on 1774 article reviews
    ab189034 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Immunostaining:

    Article Title: Joint Degeneration in a Mouse Model of Pseudoachondroplasia: ER Stress, Inflammation, and Block of Autophagy
    Article Snippet: .. Briefly, the limbs were fixed in 95% ethanol followed by decalcification in immunocal (StatLab McKinney, TX, USA) for 1 week, and pepsin (1 mg/mL in 0.1N HCl) was used for antigen retrieval for immunostaining with antibodies for human COMP (Thermofisher, Waltham, MA, USA; MA1-20221, 1:100), CHOP (Santa Cruz Dallas, TX, USA; SC-575; 1:100), interleukin 1 (IL-1) (Abcam Cambridge, United Kingdom; ab7632, 1:200), tumor necrosis factor α (TNFα) (Abcam, Cambridge, UK; ab6671, 1:200), PDI (Santa Cruz Dallas, TX, USA; SC-20132, 1:100), p16 INK4a—(Abcam Cambridge, United Kingdom; ab189034, 1:200), pS6 (Cell Signaling Technology 2215S rabbit polyclonal, 1:200), and MMP-13 (Abcam Cambridge, United Kingdom: ab39012, 1:50). ..

    Article Title: Joint degeneration in a mouse model of pseudoachondroplasia: ER stress, inflammation and autophagy blockage
    Article Snippet: .. Briefly, the limbs were fixed in: 95% ethanol followed by decalcification in immunocal (StatLab McKinney, TX) for 1 week and pepsin (1 mg/ml in 0.1N HCl) was used for antigen retrieval for immunostaining with human COMP (Thermofisher; MA1-20221, 1:100), CHOP (Santa Cruz; SC-575; 1:100), interleukin 1 (IL-1) (Abcam; ab7632, 1:200), tumor necrosis factor α (TNFα) (Abcam; ab6671, 1:200), PDI (Santa Cruz; SC-20132, 1:100), p16 INK4a - (Abcam; ab189034, 1:200), pS6 (Cell Signaling Technology 2215S rabbit polyclonal, 1:200), MMP-13 (Abcam: ab39012, 1:50) or 10% wt/vol formalin for terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate-biotin nick end (TUNEL) labeling. ..

    TUNEL Assay:

    Article Title: Joint degeneration in a mouse model of pseudoachondroplasia: ER stress, inflammation and autophagy blockage
    Article Snippet: .. Briefly, the limbs were fixed in: 95% ethanol followed by decalcification in immunocal (StatLab McKinney, TX) for 1 week and pepsin (1 mg/ml in 0.1N HCl) was used for antigen retrieval for immunostaining with human COMP (Thermofisher; MA1-20221, 1:100), CHOP (Santa Cruz; SC-575; 1:100), interleukin 1 (IL-1) (Abcam; ab7632, 1:200), tumor necrosis factor α (TNFα) (Abcam; ab6671, 1:200), PDI (Santa Cruz; SC-20132, 1:100), p16 INK4a - (Abcam; ab189034, 1:200), pS6 (Cell Signaling Technology 2215S rabbit polyclonal, 1:200), MMP-13 (Abcam: ab39012, 1:50) or 10% wt/vol formalin for terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate-biotin nick end (TUNEL) labeling. ..

    Labeling:

    Article Title: Joint degeneration in a mouse model of pseudoachondroplasia: ER stress, inflammation and autophagy blockage
    Article Snippet: .. Briefly, the limbs were fixed in: 95% ethanol followed by decalcification in immunocal (StatLab McKinney, TX) for 1 week and pepsin (1 mg/ml in 0.1N HCl) was used for antigen retrieval for immunostaining with human COMP (Thermofisher; MA1-20221, 1:100), CHOP (Santa Cruz; SC-575; 1:100), interleukin 1 (IL-1) (Abcam; ab7632, 1:200), tumor necrosis factor α (TNFα) (Abcam; ab6671, 1:200), PDI (Santa Cruz; SC-20132, 1:100), p16 INK4a - (Abcam; ab189034, 1:200), pS6 (Cell Signaling Technology 2215S rabbit polyclonal, 1:200), MMP-13 (Abcam: ab39012, 1:50) or 10% wt/vol formalin for terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate-biotin nick end (TUNEL) labeling. ..



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    TRB3 is increased in human and knee articular cartilage. ( A , B ) Blots were stripped and reprobed with β-actin as a loading control. Densitometric analysis for protein levels of TRB3, P62, <t>P16,</t> P21, Beclin and LC3B. ( C ) Articular cartilage sections of HC or OA were analyzed immunohistochemically for TRB3, P62, and P16. The results are described as the mean ± SD. *P < 0.05, **P < 0.01 vs. normal group.
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    Cell Signaling Technology Inc ab189034
    TRB3 is increased in human and knee articular cartilage. ( A , B ) Blots were stripped and reprobed with β-actin as a loading control. Densitometric analysis for protein levels of TRB3, P62, <t>P16,</t> P21, Beclin and LC3B. ( C ) Articular cartilage sections of HC or OA were analyzed immunohistochemically for TRB3, P62, and P16. The results are described as the mean ± SD. *P < 0.05, **P < 0.01 vs. normal group.
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    Danaher Inc p16 abcam cat ab189034
    TRB3 is increased in human and knee articular cartilage. ( A , B ) Blots were stripped and reprobed with β-actin as a loading control. Densitometric analysis for protein levels of TRB3, P62, <t>P16,</t> P21, Beclin and LC3B. ( C ) Articular cartilage sections of HC or OA were analyzed immunohistochemically for TRB3, P62, and P16. The results are described as the mean ± SD. *P < 0.05, **P < 0.01 vs. normal group.
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    Image Search Results


    TRB3 is increased in human and knee articular cartilage. ( A , B ) Blots were stripped and reprobed with β-actin as a loading control. Densitometric analysis for protein levels of TRB3, P62, P16, P21, Beclin and LC3B. ( C ) Articular cartilage sections of HC or OA were analyzed immunohistochemically for TRB3, P62, and P16. The results are described as the mean ± SD. *P < 0.05, **P < 0.01 vs. normal group.

    Journal: Aging (Albany NY)

    Article Title: High TRB3 expression induces chondrocyte autophagy and senescence in osteoarthritis cartilage

    doi: 10.18632/aging.204066

    Figure Lengend Snippet: TRB3 is increased in human and knee articular cartilage. ( A , B ) Blots were stripped and reprobed with β-actin as a loading control. Densitometric analysis for protein levels of TRB3, P62, P16, P21, Beclin and LC3B. ( C ) Articular cartilage sections of HC or OA were analyzed immunohistochemically for TRB3, P62, and P16. The results are described as the mean ± SD. *P < 0.05, **P < 0.01 vs. normal group.

    Article Snippet: Antibodies against TRB3 (Pro Sci; lot #8099) LC3-B (abcam; lot #ab192890), beclin1 (abcam; lot #ab210498), p16 (abcam; lot #ab189034), p21 (abcam; lot #ab107099), and p62 (abcam; lot #ab109012), matrix metalloproteinase-9 (abcam; lot #ab76003), and type II collagen (Santa Cruz Biotechnology; lot #sc-52658) were used in this study.

    Techniques: Control

    Chondrocyte senescent is dependent on TRB3. ( A , B ) Western blotting analyses of senescent markers p21, p16, type II collage and MMP-9 with β-actin as a loading control. ( C ) Representative graphs of cells stained for β-gal activity. The results are described as the mean ± SD. *P < 0.05, **P < 0.01 vs. normal group.

    Journal: Aging (Albany NY)

    Article Title: High TRB3 expression induces chondrocyte autophagy and senescence in osteoarthritis cartilage

    doi: 10.18632/aging.204066

    Figure Lengend Snippet: Chondrocyte senescent is dependent on TRB3. ( A , B ) Western blotting analyses of senescent markers p21, p16, type II collage and MMP-9 with β-actin as a loading control. ( C ) Representative graphs of cells stained for β-gal activity. The results are described as the mean ± SD. *P < 0.05, **P < 0.01 vs. normal group.

    Article Snippet: Antibodies against TRB3 (Pro Sci; lot #8099) LC3-B (abcam; lot #ab192890), beclin1 (abcam; lot #ab210498), p16 (abcam; lot #ab189034), p21 (abcam; lot #ab107099), and p62 (abcam; lot #ab109012), matrix metalloproteinase-9 (abcam; lot #ab76003), and type II collagen (Santa Cruz Biotechnology; lot #sc-52658) were used in this study.

    Techniques: Western Blot, Control, Staining, Activity Assay